Experimental validation of the core nuclear proteome by IFA - non-nuclear localization. All panels show protein localization in trophozoites. Protein acronym and PlasmoDB gene accession numbers are indicated to the left. Peptide detection or absence in individual fractions is indicated by orange or white boxes, respectively. Epitope-tagged candidates were detected using anti-HA antibodies (red). The reference compartments cytosol and ER were visualized using antibodies against GAPDH and PfBIP, respectively (green). White frames refer to the magnified view of the nuclear area shown in the rightmost images. (a) Four NuProCs do not co-localize exclusively with the DAPI-stained area of the parasite nucleus. (b) None of the six non-nuclear protein candidates (n-NuProCs) localize to the parasite nucleus, and n-NuProCs 1, 2, 4, 5, and 6 co-localize with the ER marker PfBIP.