Figure 8

Effects of decitabine and azacitidine treatment on genome rearrangement in Oxytricha trifallax. (a) Staging of 88 h exconjugant cell populations treated with azacitidine or decitabine, using criteria from Figure 2e. (b) qPCR to measure relative levels of MIC or MAC loci in the same cells staged in (a). Blue bars represent azacitidine-treated populations, red bars represent decitabine-treated populations, and grey bars are untreated controls, set to 1. All qPCR was performed in triplicate and the average is plotted ±standard error. The Student's 1-tailed t-test for unequal variance was used to measure statistical significance (*, p < 0.05; **, p < 0.01, ***, p < 0.005). (c) Replicate experiment showing reproducible accumulation of MIC DNA, and less processed MAC DNA, in azacitidine-treated exconjugants. (d) Replicate experiment showing reproductible accumulation of micronuclear MIC DNA and less MAC DNA in decitabine-treated cell populations. All labeling is as in (a). MAC, macronuclear; MIC, micronuclear; qPCR, quantitative PCR; Veg, vegetative.