Aging effects on DNA methylation modules in human brain and blood tissue

Table 1 Description of DNA methylation data sets

Set	Analysis	n	Tissue	Description	Mean age	Age range	Platform	Reference	Public availability
1	Consensus	92	WB	Dutch controls from ALS study	64	34-88	Infin 27 k	Novel data	GSE41037
2	Consensus	273	WB	Dutch controls from SZ study	33	16-65	Infin 27 k	Novel data	GSE41037
3	Consensus	293	WB	Dutch cases, SZ	34	17-86	Infin 27 k	Novel data	GSE41037
4	Consensus	190	WB	Type 1 diabetics	44	24-74	Infin 27 k	[15]	GSE20067
5	Consensus	87	WB	Healthy older women	63	49-74	Infin 27 k	[14]	GSE20236
6	Consensus	261	WB	Healthy postmenopausal women from UKOPS	65	52-78	Infin 27 k	[15, 33]	GSE19711
7	Consensus	132	FCTX	FCTX brain	48	16-101	Infin 27 k	[18]	GSE15745
8	Consensus	126	TCTX	TCTX brain	48	15-101	Infin 27 k	[18]	GSE15745
9	Consensus	123	PONS	PONS brain	46	15-101	Infin 27 k	[18]	GSE15745
10	Consensus	111	CRBLM	CRBLM brain	47	16-96	Infin 27 k	[18]	GSE15745
11	Validation	94	WB 450 k	Controls and SZ	32	18-65	Illumina 450 k	Novel data	GSE41169
12	Validation	24	MSCs	MSCs	50	21-85	Infin 27 k	[34, 35]	GSE26519+GSE17448
13	Validation	50	CD14+CD4+	CD4+ T-cells and CD14+ monocytes	36	16-69	Infin 27 k	[14]	GSE20242
14	Validation	398	Leukocyte	Pediatric population	10	3-17	Infin 27 k	[24]	GSE27097
15	Validation	72	Leukocyte	Healthy children	5	1-16	Illumina 450 k	[24]	GSE36064
16	Validation	108	Prefrontal cortex	Healthy controls	26	-0.5-84	Infin 27 k	[19]	BrainCloudMethyl

Data sets 1 to 10 were used in the consensus network analysis while data sets 11 to 16 were used in the module validation (preservation) analysis. Our novel WB DNA methylation data sets (numbered 1 to 3 and 11) are composed of (n = 92 + 273 + 293 + 94) individuals. The study involved multiple tissues (blood, brain) and different populations (adults and healthy children). Note that the mean age (and age ranges) differ greatly across the studies. ALS, amyotrophic lateral sclerosis; CRBLM, cerebellum; FCTX, frontal cortex; MSC, mesenchymal stromal cell; PONS, caudal pons; SZ, schizophrenia; TCTX, temporal cortex; UKOPS, United Kingdom Ovarian Cancer Population Study; WB, whole blood.

ISSN: 1474-760X