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Table 3 Cost comparison of RRBS and mRRBS

From: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling

  

mRRBS

RRBS

Enzymes

Total (96 samples) enzymes

$665.99

$998.69

 

Per sample

$6.94

$10.40

Other supplies and sequencinga

Total (96 samples) other supplies and sequencing

$16,770.00

$15,360.00

 

Per sample

$174.69

$160.00

With salaryb

Total (96 samples) supplies + salary

$18,820.60

$37,254.08

 

Total per sample cost

$196.05

$388.06

  1. aSequencing costs are based on the current list price for HiSeq 40 bp indexed single read at the Whitehead Institute Core Facility. bUsing the previous RRBS method, an estimated 72 days are required to complete library preparation for 96 samples, whereas only 6 days are required using the mRRBS method. Salary costs are calculated using these labor estimates with a $60,000 annual research associate salary. Values are US dollars.