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Table 1 Histone isoforms identified from 2D RP-WCX-HILIC LC-MS/MS analysis of 7

From: Enhanced top-down characterization of histone post-translational modifications

 

H4

H2B

H2A

H3

Total

RP-WCX-HILIC

105 (10)

110 (19)

77 (28)

416 (69)

708 (126)

RPLC

36 (1)

21 (0)

58 (8)

12 (1)

127 (10)

WCX-HILIC

27 (3)

64 (9)

26 (4)

18 (3)

136 (19)

  1. The total number of isoforms for each core histone family member are shown. The numbers in parentheses represent phosphorylated isoforms. RPLC, reversed phase liquid chromatography; WCX-HILIC, weak cation exchange-hydrophilic interaction liquid chromatography.