Figure 2From: Enhanced top-down characterization of histone post-translational modificationsSeparation of H4 isoforms according to degree of acetylation using WCX-HILIC. Isoforms with more acetylation carry less positive charges and, thus, have weaker electrostatic interaction with negative function groups (carboxylate for PolyC AT A) on the stationary phase and elute earlier.Back to article page