Figure 1
From: BSmooth: from whole genome bisulfite sequencing reads to differentially methylated regions
The need for biological replicates. We show smoothed methylation profiles for three normal samples (blue) and matched cancers (red) from the Hansen data [1]. Also shown is the smoothed methylation profile for an IMR90 cell-line (black) from the Lister data [3]. Had we only analyzed normal-cancer pair 3 (thick lines), there would appear to be a methylation difference between cancer and normal in this genomic region. When all three cancer-normal pairs are considered, however, this region does not appear to be a cancer-specific differentially methylated region.