Stage-dependant DNA methylation in relation to gene repression and alternative splicing. (a) Graphical representation of differential methylation profiles in the NBL-specific DNase II gene encoding protein EFV53263.1. Ad (red line) and ML (blue line) show hypermethylation in their gene upstream regions. (b) Distribution of methylation on both sides of donor and acceptor sites in 20-nucleotide sliding windows of 200 nucleotides total length centered at the splice junctions (jxn). The exon-intron boundaries are shown in vertical dashed lines. (c) Graphical representation of methylation densities in constitutive and skipped exons, introns and 100-nucleotide 5', 3' intronic sites. The relative widths of the shaded regions correspond to methylation frequencies.