SpliceGrapher prediction from RNA-Seq and EST data. This example shows how SpliceGrapher can use both RNA-Seq data and EST data to produce predictions that incorporate the strengths of each data type. RNA-Seq data provide evidence for two novel splice junctions (fourth panel down, highlighted in green) that SpliceGrapher uses to infer an alternative 3' splicing event. EST alignments provide compelling evidence for an intron retention event. SpliceGrapher combines these predictions into the final predicted graph.