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Figure 3 | Genome Biology

Figure 3

From: Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis

Figure 3

Quality assessment of the Illumina sequence data. (a) Number of reads with barcodes that passed Illumina filtering and aligned to the reference templates using Bowtie from individually indexed libraries (n = 12). Range, 641 k to 978 k reads; mean ± standard deviation, 809 k ± 107 k. (b) Percentage of total (unaligned) reads that fall into a mean Phred quality interval. Note > 80% of the reads have mean Phred quality scores ≥25. (c) Nucleotide content as a function of sequencing cycles (n = 47). Note that the nucleotide proportions closely match the expected proportions as determined from the templates.

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