Figure 3From: Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysisQuality assessment of the Illumina sequence data. (a) Number of reads with barcodes that passed Illumina filtering and aligned to the reference templates using Bowtie from individually indexed libraries (n = 12). Range, 641 k to 978 k reads; mean ± standard deviation, 809 k ± 107 k. (b) Percentage of total (unaligned) reads that fall into a mean Phred quality interval. Note > 80% of the reads have mean Phred quality scores ≥25. (c) Nucleotide content as a function of sequencing cycles (n = 47). Note that the nucleotide proportions closely match the expected proportions as determined from the templates.Back to article page