Figure 1
From: Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis
Schematic diagram of the sequencing strategy. Sample pools of 40 samples × 12 pools were generated from a cohort of 480 individuals for PCR amplification of individual exons. After blunt-ended ligation and random fragmentation, PCR amplicons from individual sample pools were used to generate indexed sequence libraries. The 12 indexed libraries were combined in equal molar amounts and sequenced in one lane of a flow cell using an Illumina GAII.