Processing steps | Number of DNA changes |
---|---|
Different from the C57BL/6J reference sequence (C3H/Drsh) | 7261538/7242100 |
C3H not same as Drsh | 5022723 |
Not in dBSNP and 17 wildtype strains | 3654870 |
Samtools quality filter | 76264 |
Mapping quality > 45 and read depth > 10 | 7980 |
Remove intronic and intergenic variants | 1260 |
Select stop, nonsynonymous and splice site SNVs | 23 |
Confirm with capillary sequencing | 1 |