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Table 2 Representative coverage statistics from exome re-sequencing (2 × 100 bp) of six mutant strains

From: Mutation discovery in mice by whole exome sequencing

  Sample
  5330 (hbck) 6246 (sunk) 8568 (lear) 12856 (shep) 13782 (aphl) 13716 (vgim)
Targeted exons 203,224 203,224 203,224 203,224 203,224 203,224
Final target bases 54,367,244 54,367,244 54,367,244 54,367,244 54,367,244 54,367,244
Target bases covered 52,934,978 52,493,811 52,832,014 52,647,881 52,664,921 53,004,900
Percentage target bases covered 97.37 96.55 97.18 96.84 96.87 97.49
Target bases not covered 1,432,266 1,873,433 1,535,230 1,719,363 1,702,323 1,362,344
Percentage target bases not covered 2.63 3.45 2.82 3.16 3.13 2.51
Total readsa 39,675,108 39,641,830 31,817,686 42,405,386 59,956,764 67,359,382
Number of reads in target regions 23,319,015 23,335,916 19,211,748 25,227,205 36,227,876 39,948,582
Percentage reads in target regions 58.77 58.87 60.38 59.49 60.42 59.31
Average coverage 32.72 32.59 26.75 35.32 50.78 56.31
Median coverage 30.33 30.02 23.23 33.02 46.61 50.02
Coverage at 20× 76.4 73.6 61.9 77.5 85.8 88
Coverage at 10× 92.1 89.3 87.1 90.7 92.9 94.5
Coverage at 5× 95.7 93.8 94.3 94.4 95.1 96.2
Coverage at 1× 97.4 96.6 97.2 96.8 96.9 97.5
NC80 0.51 0.47 0.46 0.49 0.47 0.46
1/NC80 1.94 2.13 2.18 2.06 2.13 2.17
  1. 1/NC80 is the fold 80 penalty, which represents the fold of over sequencing necessary to move 80% of the below median bases to median. Coverage statistics for all samples sequenced can be found in Additional file 3. a2 × 100 bp, Illumina HiSeq.