Copy number variation detection in three blastomere samples. (a,c,e) Genome-wide CNV detection of blastomere A (a), blastomere E (c) and blastomere G (e) from embryo 20 before channel clone normalization. (b,d,f) Genome-wide CNV detection of blastomere A (b), blastomere E (d) and blastomere G (f) from embryo 20 after channel clone normalization. The x-axis represents the coordinate range from chromosome 1 to × and the y-axis represents the log2 ratios. The blue line represents the CBS segmentation line. The green regions represent the duplication region and red regions represent the deletion region called by the CGHcall program.