Figure 6

Classes of dynamically regulated genes during planarian regeneration. (a, b) Venn diagrams depicting the number of statistically significantly regulated genes detected during the Illumina transcriptome sequencing (RNAseq) time course (adjusted P-value < 0.001, and log₂ fold change > 0.7 or < -0.7) at one or more time points. Colors indicate 0.5 to 1 h samples (blue; included only in (a)), 2 to 3 h samples (yellow), 4 to 8 h samples (purple), 10 to 18 h samples (green) and 24 to 72 h samples (red; included only in (b)). (c) Heatmap display of the consensus results from 100 k-means clustering runs with z-score transformed expression changes of significantly regulated genes (P-value and log₂ fold change cutoff as in (a)). Rows correspond to genes, columns correspond to Illumina RNAseq time points. The color scale ranges from negative (blue) through neutral (black) to positive z-scores (yellow). Genes discussed in the main text are indicated by arrowheads.