From: Hybrid selection for sequencing pathogen genomes from clinical samples
Hybrid temperature | Stringency | Wash | Pre-hybrid selection [DNA] (pg/μl) | Post-hybrid selection [DNA] (pg/μl) | Fold enrichment |
---|---|---|---|---|---|
65°C | High | 0.10 × SSC | 10.0 | 342.9 | 34.3 |
 | Med/high | 0.25 × SSC | 10.0 | 258.2 | 25.8 |
 | Med/low | 0.50 × SSC | 10.0 | 227.9 | 22.8 |
 | Low | 0.75 × SSC | 10.0 | 181.4 | 18.1 |
60°C | High | 0.10 × SSC | 10.0 | 288.6 | 28.9 |
 | Med/high | 0.25 × SSC | 10.0 | 232.9 | 23.3 |
 | Med/low | 0.50 × SSC | 10.0 | 203.5 | 20.4 |
 | Low | 0.75 × SSC | 10.0 | 196.3 | 19.6 |