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Table 2 Quantitative PCR enrichment measurements

From: Hybrid selection for sequencing pathogen genomes from clinical samples

Hybrid temperature Stringency Wash Pre-hybrid selection
[DNA] (pg/μl)
Post-hybrid selection
[DNA] (pg/μl)
Fold enrichment
65°C High 0.10 × SSC 10.0 342.9 34.3
  Med/high 0.25 × SSC 10.0 258.2 25.8
  Med/low 0.50 × SSC 10.0 227.9 22.8
  Low 0.75 × SSC 10.0 181.4 18.1
60°C High 0.10 × SSC 10.0 288.6 28.9
  Med/high 0.25 × SSC 10.0 232.9 23.3
  Med/low 0.50 × SSC 10.0 203.5 20.4
  Low 0.75 × SSC 10.0 196.3 19.6