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Table 1 Quantitative PCR enrichment measurements from 12 clinical samples

From: Hybrid selection for sequencing pathogen genomes from clinical samples

 

Percentage

 

Parasite [DNA] (pg/μl)

 

Sample

parasite DNA

WGA

Pre-hybrid selectiona

Post-hybrid selectiona

Fold enrichment

Th231.08 (round 1)

0.11

Yes

1.8 (0.6)

71.1 (5.6)

39.7

Th231.08 (round 2)

7.7

No

71.1 (5.6)

349.1 (74.9)

4.9

Th145.08

20

No

198.4 (17.4)

477.6 (66.7)

2.4

Th032.09

12

No

114.7 (2.9)

372.6 (59.3)

3.2

Th029.09

3

No

33.6 (0.8)

317.3 (54.7)

9.4

Th093.09

2.8

No

28.5 (1.5)

365.6 (53.4)

12.8

Th090.08

2.3

No

37.7 (1.1)

300.4 (46.9)

8.0

Th139.08

2.1

No

23.6 (0.6)

346.2 (50.7)

14.7

Th197.08

1.1

No

14.6 (0.0)

222.7 (36.1)

15.3

Th140.08

0.99

No

9.6 (0.1)

251.5 (37.4)

26.2

Th190.08

0.64

No

5.1 (0.2)

218.7 (34.0)

43.2

Th238.08

0.53

No

6.7 (0.2)

273.4 (38.1)

41.0

Th127.09

1.6

No

26.8 (0.4)

368.5 (57.1)

13.7

Th175.08

48

Yes

275.8 (7.2)

556.9 (79.4)

2.0

  1. aNumbers in parentheses represent standard deviations. WGA, whole genome amplification.