Genome-wide sequencing coverage and composition. (a) Coverage thresholds for unamplified (green) and WGA (purple) whole genome baits compared to pure P. falciparum (gray) and simulated coverage from a non-hybrid-selected mock clinical sample (yellow). (b) Genome-wide coverage as a function of %GC. The vertical black line represents average exonic %GC. The red histogram represents the probability density distribution of genome composition (right vertical axis). Lines depict coverage (left vertical axis) of pure P. falciparum DNA (gray), as well as unamplified (green) and WGA (purple) hybrid-selected samples initially containing 1% P. falciparum DNA. %GC, the percentage of nucleotides in the genome which are G or C.