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Figure 2 | Genome Biology

Figure 2

From: Single cell transcriptomics of neighboring hyphae of Aspergillus niger

Figure 2

Amplification of cDNA from the tips of five single neighboring hyphae. (a-c) Apical regions of 200 μm of hyphae at the periphery of sandwiched colonies were selected by using the measuring (a) and drawing (b) tool. Fragments of a single hypha were catapulted into a cap of an Eppendorf tube by using the autoLPC option (c). RNA of the single hyphae was isolated, converted into cDNA and amplified. (d) The amplified cDNA was analyzed with a Bioanalyser. The electrophoresis gel image (nt, nucleotides; L, ladder) and the electropherogram (y-axis represents fluorescence units (FU); x-axis represents run time in seconds (s)) are given for the five samples. Amplicons of hyphae 1 to 3 and hyphae 4 and 5 were mainly 50 to 100 bp and 100 to 300 bp in length, respectively.

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