Figure 4

EMSA-Seq profiling of the NF-κB RELA-containing dimers. (a) Grouping of 11-mer sequences bound by the homodimer RELARELA and the heterodimers RELAp50 and RELAp52 during EMSA-Seq. In parentheses are proportions out of all possible 2,097,152 11-mer sequences. (b) De novo motif identification was performed on the 50 and 1,000 top-scoring 11-mer sequences from each experiment using the Priority algorithm [51]. No priors were used for motif identification and logos were generated using the enoLOGOS web tool [52]. For every dimer, the percentage proportion of sequences that are non-canonical (MATCH < 0.75) and that have contributed towards construction of the motif has been indicated.