Figure 4

The miR-30 family positively regulates hMADS cell adipocyte differentiation. (a) Sub-confluent hMADS cells were transfected with one of anti-miR control (anti-neg), anti-miR 30, pre-miR control (pre-miR-neg), pre-miR-30a, or pre-miR-30d and were induced to undergo adipocyte differentiation 3 days later. Differentiation was assessed at the indicated time points (D4 or D10) by photomicrographic recording (top row) and Oil red O plus crystal violet counter-staining (lower row). (b) Assessment of adipogenesis by GPDH enzymatic activity. Results are means of three culture wells (24-well plates). Error bars represent mean ± standard error of the mean (N = 3). *P < 0.05. (c) Expression of adipogenesis-induced genes (CEBPβ, PPARγ and FABP4) by qPCR. The level of expression of each gene in control cells (anti-miR-neg or pre-miR-neg) was taken as 1.