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Figure 1 | Genome Biology

Figure 1

From: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis

Figure 1

Distribution of deep-sequenced small RNAs across non-coding RNA categories. (a) Reads were matched versus the hg19 genome build and then distributed in an exclusive manner to human miRNAs, as well as miRNAs of species other than human (mirBase 16), to UCSC annotated sequences (UCSC Refflat file) and finally to non-coding RNA classes (fRNAdb, database of ncRNA.org): piwi-interacting RNA (piRNA), tRNA, rRNA, small nucleolar RNA (snoRNA) and other non-coding RNA (ncRNA). Reads that did not match any of those non-coding RNA classes were labeled as 'non-annotated'. Data are the average of read sequencing frequency (percentage) for each experimental condition. ND, undifferentiated cells; AD3, adipogenesis day 3; AD8, adipogenesis day 8. (b) Relative abundance of reads corresponding to the 30 most expressed miRNAs in undifferentiated hMADS cells. Read counts are normalized to 106 total miRNA reads per sample. Data are the average of sequencing of samples from two independent experiments, each with two technical replicates with opposite sequencing directions (error bars represent ± standard error).

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