Figure 4

RACE suggests two long telomere-associated transcripts are transcribed in the TARE 2-3 region. 3' RACE products of seven distinct lncRNA-TARE sequences terminated at a precise syntenic site on either side of TARE 2 (asterisks). Taken together with the 5' RACE product suggests two species approximately 1.5 kb and 3.1 kb in length are transcribed towards the telomeres from the TARE 3 boundary. Genome-wide, the palindromic SPE2 DNA motif (green triangles) is enriched only in the TARE 2-3 region from which lncRNA-TARE is transcribed and in the upstream promoter element of upsB-type var genes. Primers are depicted as black triangles and numbers represent base-pair coordinates corresponding to the left end of chromosome 4. SPE2, subtelomeric var gene promoter element 2.