From: The mouse genetics toolkit: revealing function and mechanism
ES cell line | Genetic background | Comments | Reference |
---|---|---|---|
E14TG2a | 129P2OlaHsd | Feeder-independent; suitable for injections into C57BL/6 blastocysts | [104] |
AB2.2 | 129S7/SvEvBrd-Hprtb-m2 | Feeder-dependent | [105] |
J1 | 129SvJae | Feeder-dependent | [106] |
Bruce4 | C57BL/6J-Thy1.1 | Have a tendency to aneuploidy | [107] |
B6/Blu-1 | C57BL/6N | Generated by Tim Ley (Washington University, St Louis, USA) | Personal communication |
JM8.parental | C57BL/6N | 76% GLT rate | [31] |
JM8.F6 | C57BL/6N | Feeder-dependent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/c blastocysts | [31] |
JM8.N4 | C57BL/6N | Feeder-independent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/cblastocysts | [31] |
JM8A1.N3 | C57BL/6N | JM8.F6-derived line with a repaired Agouti locus. Feeder independent | [31] |
C2 | C57BL/6NTac | Efficient GLT using a combination of ICR morula aggregation with outbred host embryos | [108] |
R1 | 129X1/SvJ × 129S1 (hybrid) | Feeder-dependent | [109] |
G4 | 129S6/SvEvTac × C57BL/6Ncr F1 hybrid | Feeder-dependent; typically used for tetraploid complementation assays | [75] |