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Table 1 Commonly used ES cell lines for generating genetically modified mice

From: The mouse genetics toolkit: revealing function and mechanism

ES cell line Genetic background Comments Reference
E14TG2a 129P2OlaHsd Feeder-independent; suitable for injections into C57BL/6 blastocysts [104]
AB2.2 129S7/SvEvBrd-Hprtb-m2 Feeder-dependent [105]
J1 129SvJae Feeder-dependent [106]
Bruce4 C57BL/6J-Thy1.1 Have a tendency to aneuploidy [107]
B6/Blu-1 C57BL/6N Generated by Tim Ley (Washington University, St Louis, USA) Personal communication
JM8.parental C57BL/6N 76% GLT rate [31]
JM8.F6 C57BL/6N Feeder-dependent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/c blastocysts [31]
JM8.N4 C57BL/6N Feeder-independent JM8 subline. Suitable for injections in BALB/c or C57BL/6J-Tyrc/cblastocysts [31]
JM8A1.N3 C57BL/6N JM8.F6-derived line with a repaired Agouti locus. Feeder independent [31]
C2 C57BL/6NTac Efficient GLT using a combination of ICR morula aggregation with outbred host embryos [108]
R1 129X1/SvJ × 129S1 (hybrid) Feeder-dependent [109]
G4 129S6/SvEvTac × C57BL/6Ncr F1 hybrid Feeder-dependent; typically used for tetraploid complementation assays [75]
  1. Abbreviations: GLT, germ line transmission; ICR, Institute for Cancer Research.