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Table 1 Comparison of EMIRGE with de novo assembly

From: EMIRGE: reconstruction of full-length ribosomal genes from microbial community short read sequencing data

Method Community Number of expected SSUs Number of SSU sequences or fragments Number classified at genus levela N50 Weighted UniFrac distance
EMIRGE Simulated simple 10 11 10 (90%) 1,537 0.03
Assembly fragments Simulated simple 10 78 39 (50%) 383 0.32
EMIRGE Simulated complex 52b 23 20 (87%) 1,488 0.04
Assembly fragments Simulated complex 52b 320 212 (66%) 122 0.26
EMIRGE Natural Unknown 11 9 (82%) 1,484 NA
Assembly fragments Natural Unknown 137 85 (62%) 182 0.32c
  1. aRDP classifier with bootstrap cutoff > 50%; eukaryotes not classified. bWith abundance > 0.5%. cDistance to EMIRGE-defined community. NA, Not Applicable: true community structure is unknown