Figure 6

Effect of sequencing library characteristics on EMIRGE performance. The effects of sequencing effort (x axis), read length, and insert size were evaluated by running EMIRGE on the complex community with varying input. Reconstructed communities were compared to the expected community with the weighted UniFrac distance metric [30]. For the varying insert size experiment, a single sequencing effort was chosen (76-bp read length; 80,000 genomic reads; see Materials and methods).