DNA sequence conservation of DXZ4. (a) Schematic map of a single human 3-kb DXZ4 monomer represented by an open arrow. Distance in kilobases is given along the top. Underneath, the colored bars indicate the regions of DXZ4 corresponding to the defined promoter, CTCF and TFIID binding sites  and the 402-bp interval of DXZ4 that is most conserved in primates. (b) Phylogenetic tree showing inferred evolutionary relationship of DXZ4 in primates. Sequence alignments were made against a 402 nucleotide region of human DXZ4 that is most conserved in all primates (nucleotides 517 to 918 of accession number [GenBank:HQ659140]). Percentage nucleotide identity is indicated to the far right. Primates are color-coded as follows: blue, great apes; pink, lesser apes; yellow, Old World monkeys; brown, New World monkeys; red, lemurs; green, galago; black, tarsier. The tree image was generated using MUSCLE version 3.8 . (c) Predicted higher-order organization of the ring-tailed lemur DXZ4 sequence as revealed by dot-plot analysis. A single 3-kb DNA sequence is on the y-axis, whereas approximately 70 kb of BAC clone LB2-162N9 is given on the x-axis. The dot-plot was generated using the default settings for NCBI Blast2, and the output image labeled in Adobe Photoshop CS2.