Motifs enriched in recombination hotspot sequences. Motifs were identified from hotspot sequences using anr (any number of repetitions) in MEME. (a) A 21-bp motif from subtelomeric hotspot sequences of the 7G8 × GB4 cross that is similar to that of the Rep20 repeat reported previously [21–23]. (b) A GC-rich 12-bp repeat from subtelomeric hotspot sequences of the 7G8 × GB4 cross. (c) A 12-bp G/C-rich motif from the non-subtelomeric combined hotspot sequences of both crosses. (d,e) Two 12-bp G/C-rich motifs from subtelomeric and nonsubtelomeric sequences with at least one crossover within 5-kb interval, respectively, from the 7G8 × GB4 cross.