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Figure 1 | Genome Biology

Figure 1

From: Mapping meiotic breaks: Spo11 oligonucleotides precisely mark the spots

Figure 1

Meiotic DSB formation and genomic approaches to DSB mapping in budding yeast. The pathway of meiotic DSB formation and processing is shown together with the genetic tools and genomic techniques used to map DSB sites. (a, b) Spo11 catalyzes the formation of DSBs. (b) In rad50S budding yeast mutants further processing is blocked so Spo11 remains attached to DSB-ends [3]. Microarrays can be used to analyze these Spo11-associated DNA fragments. (c) Pan et al. [1] isolated Spo11-oligonucleotide complexes from wild-type budding yeast and used deep sequencing to precisely identify DSB locations and quantify their relative activities. (d) Another approach [6, 7] is to enrich the single-stranded DNA formed at processed DSB ends and use this material as a microarray probe. In this case the dmc1 mutation is used to block DSB repair. See text for more details.

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