Figure 5

A T-DNA allele of ebi-2 that results in a reduction in EBI expression and crossing out the PRR7 SNP result in similar clock phenotypes to ebi-1 , supporting that the circadian phenotype of ebi-1 is due to a SNP in At5g05660. Transgenic seedlings carrying the LUC reporter gene fused to the CAB2 promoter were entrained under 12-h light/12-h dark cycles for 7 days, after which luminescence was monitored in either constant darkness or constant red light. (a) Analysis of CAB2 activity under constant red light at 22°C in: ebi-1-clean-1, the ebi-1 mutant with a WT PRR7 gene (closed triangles); the ebi-1 mutant (closed squares); prr7-clean-1, the prr7 mutant with WT ebi-1 (open triangles) and WT Ws-2 (open squares). (b) Analysis of CAB2 activity under constant red light at 22°C in ebi-2 (closed squares) and WT Col-0 (open squares). (c) Analysis of CAB2 activity under constant darkness at 22°C in ebi-2 (closed squares) and WT Col-0 (open squares). (d) EBI expression is reduced in the ebi-2 mutant. RNA expression levels of EBI relative to β-tubulin were measured at either 1 h or 13 h under 12-h light/12-h dark cycles in both WT (white columns) and ebi-2 (gray columns).