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Table 4 Effects of IQ-143 on diverse enzymes of oxidative phosphorylation and energy and nucleotide metabolism of S. aureus USA300 and S. epidermidis RP62A

From: Modeling antibiotic and cytotoxic effects of the dimeric isoquinoline IQ-143 on metabolism and its regulation in Staphylococcus aureus, Staphylococcus epidermidisand human cells

  Concentration of IQ-143 (μM)b
Enzymea 0.00 0.16 1.25
S. aureus USA300    
   OP_complex1 0.0396 0.0260 0.0310
   OP_complex2 0.0396 0.0260 0.0310
   [OP_complex3] 0.0791 0.0520 0.0619
   OP_complex4 0.0396 0.0260 0.0310
   OP_complex5 0.0214 0.0109 0.0031
   PurM_DNA-directed-RNA-polymerase_ATP 0.0791 0.0000 0.0000
   PurM_DNA-directed-RNA-polymerase_CTP 0.0396 0.0260 0.0310
   PurM_DNA-directed-RNA-polymerase_GTP 0.0396 0.0260 0.0310
   PurM_DNA-directed-RNA-polymerase_UTP 0.0285 0.0229 0.0285
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dATP] 0.0396 0.0260 0.0121
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dCTP] 0.0396 0.0260 0.0310
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dGTP] 0.0396 0.0260 0.0310
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dTTP] 0.0396 0.0260 0.0310
   [SERP0841-PurM_PNPase_ADP] 0.0791 0.0520 0.0619
   [SERP0841-PurM_PNPase_GDP] 0.0265 0.0174 0.0207
   Glyc_glyceraldehyde-3-P-dehydrogenase_NAD+ 0.1582 0.1040 0.1238
   Glyc_glyceraldehyde-3-P-dehydrogenase_NADP+ 0.0601 0.2102 0.1251
   TCA_pyruvate_dehydrogenase 0.0396 0.0260 0.0310
S. epidermidis RP62A    
   OP_complex1 0.0201 0.0201 0.0126
   OP_complex2 0.0161 0.0161 0.0050
   [OP_complex3] 0.0361 0.0361 0.0175
   OP_complex4 0.0334 0.0334 0.0292
   OP_complex5 0.0669 0.0669 0.0585
   PurM_DNA-directed-RNA-polymerase_CTP 0.0334 0.0334 0.0292
   PurM_DNA-directed-RNA-polymerase_GTP 0.0120 0.0120 0.0436
   PurM_DNA-directed-RNA-polymerase_UTP 0.0334 0.0334 0.0292
   PurM_DNA-directed-RNA-polymerase_ATP 0.0334 0.0334 0.0292
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dATP] 0.0334 0.0334 0.0766
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dCTP] 0.0224 0.0224 0.0196
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dGTP] 0.0334 0.0334 0.0292
   [SERP0831-PurM_DNA-directed-DNA-polymerase_dTTP] 0.0468 0.0468 0.0409
   [SERP0841-PurM_PNPase_ADP] 0.0669 0.0669 0.0585
   [SERP0841-PurM_PNPase_GDP] 0.0120 0.0120 0.0050
   Glyc_glyceraldehyde-3-P-dehydrogenase_NAD+ 0.0669 0.0669 0.0585
   Glyc_glyceraldehyde-3-P-dehydrogenase_NADP+ 0.0241 0.0241 0.0228
   TCA_pyruvate_dehydrogenase 0.0161 0.0161 0.0468
  1. This table lists the effects of three different concentrations of IQ-143 on the activity of diverse enzymes of the described pathways and reactions in S. aureus USA300 and S. epidermidis RP62A. aEnzymes in brackets were also detected by their gene expression change in S. epidermidis RP62A. bConcentrations tested were no IQ-143, 0.16 μM IQ-143 and 1.25 μM IQ-143. PurM, purine metabolism.