Variable technical replicates. Results of correlation tests showing improvement after bias correction for technical replicates. Fraction Explained Discrepancy was calculated by dividing the change in R2 after bias correction by the difference of the initial R2 from one (a perfect correlation). Note that when two RNA-Seq datasets are compared, the correction in the legend was applied to both. The pairwise correlations of the four SRA010153 replicates versus qRT-PCR and SRA008403, respectively, were averaged for the figure. Even though the same RH priming protocol was used in both labs, the bias differs slightly (see Figure S2 of Additional file 1) between the preps, which is why our correction method was able to improve the correlation.