Figure 1

A phylogenetic tree of human TRP channels. Sequence homology analyses show that all TRP channels fall into seven subfamilies that comprise proteins with distinct channel properties. Because TRPC2 is a pseudogene in human and TRPNs are not present in mammals, we used mouse TRPC2 (ENSMUSP00000102562) and fish TRPN1 (ENSDARP00000093955) to show relations between all subfamilies. Protein sequences were aligned using ClustalW2 at the EMBL-EBI server. Phylogenetic distances were calculated using PAM matrix and the unrooted tree was obtained using NJplot [102]. The TRP subfamilies are represented by different colors. The scale bar represents 0.2 substitutions. Ensembl protein IDs for human protein sequences used in the analysis are as follows: TRPM1, ENSP00000380897; TRPM2, ENSP00000381023; TRPM3, ENSP00000350140; TRPM4, ENSP00000252826; TRPM5, ENSP00000387965; TRPM6, ENSP00000354006; TRPM7, ENSP00000320239; TRPM8, ENSP00000323926; TRPV1, ENSP00000174621; TRPV2, ENSP00000342222; TRPV3, ENSP00000301365; TRPV4, ENSP00000261740; TRPV5, ENSP00000265310; TRPV6, ENSP00000352358; TRPC1, ENSP00000273482; TRPC3, ENSP00000368966; TRPC4, ENSP00000369003; TRPC5, ENSP00000262839; TRPC6, ENSP00000340913; TRPC7, ENSP00000426070; TRPML1, ENSP00000264079; TRPML2, ENSP00000359640; TRPML3, ENSP00000304843; TRPP2, ENSP00000237596; TRPP3, ENSP00000325296; TRPP5, ENSP00000290431; TRPA1, ENSP00000262209.