From: Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
PCR condition | P. falciparum | E. coli | R. sphaeroides | 'PER' |
---|---|---|---|---|
Phusion HF short (standard) denaturation, fast ramp | 41% | 0.59% | 95% | 42% |
Phusion HF long denaturation, 2M betaine | 45% | 0.00011% | 0.0096% | 33% |
AccuPrime Taq HiFi long denaturation, extension at 65°C | 20% | 0.00015% | 0.032% | 14% |
AccuPrime Taq HiFi long denaturation, extension at 60°C | 8.8% | 0.00017% | 0.085% | 6.4% |