Skip to main content

Table 1 Percentage of bases covered at less than one-tenth of the mean 'PER'-wide coverage

From: Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

PCR condition P. falciparum E. coli R. sphaeroides 'PER'
Phusion HF short (standard) denaturation, fast ramp 41% 0.59% 95% 42%
Phusion HF long denaturation, 2M betaine 45% 0.00011% 0.0096% 33%
AccuPrime Taq HiFi long denaturation, extension at 65°C 20% 0.00015% 0.032% 14%
AccuPrime Taq HiFi long denaturation, extension at 60°C 8.8% 0.00017% 0.085% 6.4%