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Figure 2 | Genome Biology

Figure 2

From: Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

Figure 2

Effect of temperature ramp rates. The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on thermocycler 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).

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