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Table 2 Knock-out mutants, their TraSH ratios, phenotypic predictions and knockout phenotypes

From: Interrogation of global mutagenesis data with a genome scale model of Neisseria meningitidis to assess gene fitness in vitro and in sera

   

Initial growth on CAB

Growth on MM agar

NMB

Function

Gene

TraSH prediction

TraSH ratio

Experimental phenotype

TraSH prediction

Change in TraSH ratio

Experimental phenotype

ΔNMB1646

Putative hemolysin III

 

Non-ess

0.74

Non-ess

Non-ess

1.40

Non-ess

ΔNMB0696

ABC transporter ATP-binding protein

 

Non-ess

0.495

Non-ess

Ess

-1.62

Non-ess

ΔNMB0719

Queuine tRNA-ribosyltransferase

tgt

Non-ess

1.25

Non-ess

Ess

-3.00

Ess

ΔNMB1338

Putative hydrolase

 

Non-ess

-0.034

Non-ess

Ess

-1.67

Ess

ΔNMB1277

Putative transporter

 

Non-ess

-0.0625

Non-ess

Ess

-1.62

Ess

ΔNMB1295

Formamidopyrimidine-DNA glycosylase

mutM

Non-ess

-0.174

Non-ess

Ess

-1.49

Ess

ΔNMB0318

Multidrug resistance protein A

emrA

Non-ess

2.28

Non-ess

Ess

-3.29

Ess

ΔNMB2061

Phosphoenolpyruvate carboxylase

ppc

Non-ess

1.52

Non-ess

Ess

-1.83

Ess

ΔNMB0951

Succinate dehydrogenase

sdhB

Non-ess

1.10

Non-ess

Ess

-3.13

Ess

  1. A number of knockout mutants were generated by insertional inactivation and their ability to grow on Columbia Agar Base (CAB) and minimal media agar (MM) assessed. These knockout phenotypes were compared to TraSH predicted phenotypes. A TraSH ratio of less than -0.738 is indicative of essentiality in the initial selection. Due to low transformation efficiencies when plating transformants onto MM it was not possible to determine whether ΔNMB1203 was viable on MM. TraSH ratios are the average of five biological replicates (three for sera).