Figure 2

Functional group essentiality. The percentage of genes in each functional group shown to be essential by TraSH during the initial library selection. The origin is set to 26%, the percentage of essential genes in the whole genome. Cell envelope genes include those involved in peptidoglycan, capsule and lipopolysaccaride (LPS) synthesis as well as pili; cell processes includes genes associated with cell division, pathology, and so on; intermediate metabolism includes phosphate and sulfur metabolism; energy metabolism includes glycolysis, the pentose phosphate pathway, the tricarboxylic acid cycle, fermentation, electron transport and ATP synthesis; mobile elements includes prophage-, plasmid- and transposon-associated genes; other categories are self-explanatory.