Figure 6
From: MicroRNAs and their isomiRs function cooperatively to target common biological pathways
IsomiRs target similar molecules to their canonical miRNAs. (a) Schematic diagram depicting the laboratory workflow for the pull-down of mRNA targets of a synthetic biotinylated miRNA. (b) The miRNA expression profiles of the two miRNAs and corresponding shifted isomiRs selected for pull-down experiments. (c) A four-way Venn diagram depicting the overlap between the target genes detected by microarray analysis of pulled-down fractions. (d) The distribution of the number of miRNAs/isomiRs that target genes from significantly enriched pathways. There are more genes targeted by three or more isomiRs than would be expected by chance, and less genes targeted by two or fewer isomiRs than would be expected by chance. (e) A four-way Venn diagram displaying the specific targeting of the genes used in (d).