Figure 5
From: MicroRNAs and their isomiRs function cooperatively to target common biological pathways
IsomiRs are associated with translational machinery. (a) Schematic diagram showing the laboratory workflow to isolate total miRNA, miRNA associated with mRNA, and miRNAs associated with polysomal mRNA. (b, c) Correlation scatter plots describing miRNA expression in the three preparations described in (a). The noise threshold was set at 25 tpm for these libraries. (b) Correlation scatter plot between miRNA associated with mRNA (x-axis), and miRNA associated with polysomal mRNA (y-axis). The dashed diagonal line represents perfect correlation, while the horizontal and vertical lines represent the likely noise thresholds for these data. (c) Correlation scatter plot between total cellular miRNAs, and polysomal associated miRNA. The dashed diagonal line represents perfect correlation, while the horizontal and vertical lines represent the likely noise thresholds for these data. (d) A sequence logo profile for hsa-miR-31 showing similar profiles for all three HeLa miRNA-seq samples. This example shows an ac-pre-miRNA candidate hairpin with no change in the proportion of intermediates in the translational machinery. (e) Proportional bar graph illustrating the similar distribution of isomiR categories in each of the three HeLa miRNA-seq samples. CHX, cycloheximide, nt, nucleotide.