Figure 1

Correlation between miRNA-seq, qRT-PCR, and microarrays for placental miRNAs. Different alignment strategies will produce different correlations due to the characteristics of the validation technology. (a) The Agilent miRNA microarray typically will capture signal from isomiRs due to cross-hybridization, and therefore has a higher correlation to the recursive matching strategy, which identifies both miRNAs and isomiRs. (b) TaqMan LDA qRT-PCR is more sensitive to the exact sequence of the miRNA, and therefore has higher correlations to the adaptor trimming strategy, which was filtered for exact matches to canonical miRNA sequences. In all plots, points represent mean detection values, and bi-directional error bars represent standard error. ρ values indicated are Spearman correlations. These comparisons demonstrate that the miRNA-seq technology and our analysis approach are both sensitive and specific.