Validation of microarray results and overlap between embryonic and larval gene sets. (a) Correlation between results obtained by microarray and quantitative RT-PCR (qRT-PCR) for selected genes in embryos and L3 larvae. For qRT-PCR expression values, data are the mean of three independent biological replicas. Error bars indicate standard deviation. pmp-3 and cdc-42 were used for normalization and the RNA level of each gene of interest was normalized to the mean of both reference genes. (b) Venn diagram showing overlap between up- and down-regulated genes in embryonic and L3 larval stages.