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Table 3 Filtering of smORFs with evidence of translation

From: Hundreds of putatively functional small open reading frames in Drosophila

   Pipeline filters
Gene name [reference] Length (codons) tBLASTn E-value (pass if < 10-3) Ka/Ks (pass if < 0.1) Syntenic to D. pseudoobscura RNASeq overlaps (pass if > 1)
Akh [63] 79 2e-22 0.04 False 12
CG13551 [64] 87 6e-14 0.18 False 12
CG14235 [64] 96 5e-42 0.06 True 12
CG17127 [64] 98 4e-42 0.05 True 10
CG30415 [65] 82 4e-36 0.05 False 12
CG32039 [64] 82 1e-15 0.06 False 12
CG3321 [64, 65] 81 6e-23 0.05 True 12
Cype [65] 77 8e-26 0.18 True 12
Dbi [64] 86 7e-39 0.48 True 4
Dro [66] 64 4e-17 0.32 True 9
Drs [66] 70 NA NA Na 9
IM1 [66] 45 1e-12 0.09 True 9
IM2 [63, 66] 45 9e-14 0.00 True 9
IM4 [63, 66] 42 0.075 0.11 False 12
l(2)06225 [64, 65] 99 3e-45 0.00 True 10
Mtk [66] 52 7e-11 0.27 True 12
Nplp2 [63] 86 8.4 0.07 False 11
Nplp3 [63] 90 1e-10 0.14 False 7
Nplp4 [63] 64 NA NA NA 12
RpL38 [67] 70 8e-34 0.00 False 8
RpS21 [67] 83 8e-36 0.02 True 12
RpS27 [67] 84 2e-44 0.00 False 12
RpS28b [64, 65] 65 6e-23 0.00 True 12
smt3 [64] 90 2e-47 0.00 False 12
sun [64] 61 1e-16 0.16 True 12
Number of smORFs passing each pipeline filter (out of a total of 25) 21 15 13 25
  1. Filtering of previously annotated smORFs with proteomics evidence of translation. References cited in the first column include evidence of translation for each of these smORFs. Entries in bold do not pass the corresponding filter. Ka/Ks, ratio of non-synonymous (Ka) to synonymous (Ks) nucleotide substitution; NA, not available.