Hundreds of putatively functional small open reading frames in Drosophila

Table 3 Filtering of smORFs with evidence of translation

		Pipeline filters
Gene name [reference]	Length (codons)	tBLASTn E-value (pass if < 10^-3)	Ka/Ks (pass if < 0.1)	Syntenic to *D. pseudoobscura*	RNASeq overlaps (pass if > 1)
Akh [63]	79	2e-22	0.04	False	12
CG13551 [64]	87	6e-14	0.18	False	12
CG14235 [64]	96	5e-42	0.06	True	12
CG17127 [64]	98	4e-42	0.05	True	10
CG30415 [65]	82	4e-36	0.05	False	12
CG32039 [64]	82	1e-15	0.06	False	12
CG3321 [64, 65]	81	6e-23	0.05	True	12
Cype [65]	77	8e-26	0.18	True	12
Dbi [64]	86	7e-39	0.48	True	4
Dro [66]	64	4e-17	0.32	True	9
Drs [66]	70	NA	NA	Na	9
IM1 [66]	45	1e-12	0.09	True	9
IM2 [63, 66]	45	9e-14	0.00	True	9
IM4 [63, 66]	42	0.075	0.11	False	12
l(2)06225 [64, 65]	99	3e-45	0.00	True	10
Mtk [66]	52	7e-11	0.27	True	12
Nplp2 [63]	86	8.4	0.07	False	11
Nplp3 [63]	90	1e-10	0.14	False	7
Nplp4 [63]	64	NA	NA	NA	12
RpL38 [67]	70	8e-34	0.00	False	8
RpS21 [67]	83	8e-36	0.02	True	12
RpS27 [67]	84	2e-44	0.00	False	12
RpS28b [64, 65]	65	6e-23	0.00	True	12
smt3 [64]	90	2e-47	0.00	False	12
sun [64]	61	1e-16	0.16	True	12
Number of smORFs passing each pipeline filter (out of a total of 25)		21	15	13	25

Filtering of previously annotated smORFs with proteomics evidence of translation. References cited in the first column include evidence of translation for each of these smORFs. Entries in bold do not pass the corresponding filter. Ka/Ks, ratio of non-synonymous (Ka) to synonymous (Ks) nucleotide substitution; NA, not available.

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