Figure 8

HSF occupancy within Alus in the proximal region of antisense transcripts. The possibility that putative high-score HSF sites within Alus drive antisense transcription was confirmed by ChIP-PCR using ChIP DNA made against HSF1. In response to heat shock, we observed enriched binding of HSF1 at its predicted site compared to the untreated state. The absence of a band in the 'no antibody' (No Ab) control points towards conditional and specific binding of HSF1 to the putative site.