Skip to main content
Figure 2 | Genome Biology

Figure 2

From: Heat shock factor binding in Alu repeats expands its involvement in stress through an antisense mechanism

Figure 2

Location of validated heat shock factor sites within Alus in the promoter region of up-regulated genes. A substantial fraction of up-regulated genes harbor high score (≥8.7) HSF sites within Alus in the upstream region. All the validated genes (SPINK6, HIST1H4A and ANKRD33) have their first HSF site within Alus in the promoter region from the transcription start site. Functional validation of such HSF sites through cloning, site-directed mutagenesis, transient transfection in a cell line and chromatin immunoprecipitation (ChIP) followed by PCR confirmed their role in the heat shock response.

Back to article page