Figure 5

Mean quality scores of correct and wrong bases sequenced (PhiX-95nt data) at error-prone positions and all other positions in the PhiX reference. The bases covering 161 positions of significantly elevated error rates (A, B) in the PhiX reference show lower average quality scores compared to the bases covering other positions (C, D). This is true for correctly called bases (A, C) as well as for incorrectly called bases (B, D).