Regulatory network involving 12 two component systems. These 12 RRs (each colored differently), including three non-σ54 RRs (shown on the right-hand side), are connected by their overlapping targets. DVU0946 and DVU0539 are paralogs with similar targets and binding sites and are colored the same. Objects are not drawn to scale. This is a conservative list of the most likely targets (for a more comprehensive list of DAP-chip peaks see Table S4 in Additional file 1). Where present, predicted σ54-dependent promoters are indicated. Note that there is a missing gene annotation downstream of DVU0653 (Figure S1 in Additional file 3). Validated binding site motifs (vertical bars) are colored according to the RR gene color. For RR DVU1083, only those targets with a binding site motif are shown in the figure. DBD, DNA binding domain; HK, histidine kinase; MCP, methyl-accepting chemotaxis protein; mp, membrane protein; REC, receiver domain; reg, regulator, RR, response regulator; Tr, transporter; HD, EAL, GGDEF and UspA, protein domain names. Gene names are italicized (Table S5 in Additional file 1).