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Table 1 Purple Kush sequencing library statistics
| Library | Insert size (bp) | Raw No. | Raw nt (Gb) | Filtered No. | Filtered nt (Gb) | % in a) assembly |
|---|---|---|---|---|---|---|
| Genomic DNA, Illumina 2 × 100 bp paired-end reads | ||||||
| CS-PK_SIL-1a | 181 | 143,951,601 | 28.0 | 124,499,863 | 23.8 | 82.2 |
| CS-PK_SIL-1b | 195 | 111,106,936 | 22.2 | 98,124,711 | 19.0 | 82.4 |
| CS-PK_SIL-2a | 313 | 93,774,355 | 18.8 | 81,421,333 | 15.3 | 84.7 |
| CS-PK_SIL-3b | 362 | 66,932,319 | 13.4 | 60,519,955 | 11.6 | 82.8 |
| CS-PK_SIL-B | 664 | 95,648,778 | 19.1 | 49,550,098 | 9.2 | 85.1 |
| CS-PK_SIL-C | 580 | 101,329,142 | 20.3 | 72,977,620 | 13.6 | 87.1 |
| Genomic DNA, Illumina 2 × 44 bp mate pair reads | ||||||
| CS-PK_2 kb-1a | 1,926 | 36,057,086 | 3.2 | 24,688,690 | 2.2 | 75.0 |
| CS-PK_2 kb-1b | 1,846 | 32,385,628 | 2.8 | 24,405,458 | 2.1 | 76.8 |
| CS-PK_2 kb-2a | 1,850 | 37,761,064 | 3.3 | 29,927,921 | 2.6 | 75.5 |
| CS-PK_2 kb-2b | 1,787 | 37,111,622 | 3.3 | 28,744,604 | 2.5 | 77.1 |
| CS-PK_5 kb-1 | 4,721 | 36,182,230 | 3.2 | 27,377,398 | 2.4 | 77.5 |
| CS-PK_5 kb-2 | 4,585 | 64,613,144 | 5.7 | 50,712,974 | 4.4 | 79.9 |
| Genomic DNA, 454 mate pairs | ||||||
| CS-PK_8 kb-1 | 8,000 | 557,443 | 0.20 | 192,483 | 0.069 | 77.0 |
| CS-PK_8 kb-2 | 8,000 | 484,033 | 0.17 | 176,405 | 0.063 | 74.5 |
| CS-PK_8 kb-3 | 8,000 | 603,780 | 0.21 | 221,616 | 0.079 | 78.6 |
| CS-PK_13 kb-1 | 13,000 | 430,642 | 0.11 | 96,503 | 0.030 | 75.1 |
| CS-PK_20 kb-1 | 20,000 | 611,986 | 0.19 | 216,379 | 0.070 | 77.0 |
| CS-PK_20 kb-2 | 20,000 | 575,618 | 0.21 | 228,811 | 0.081 | 77.0 |
| CS-PK_30 kb-1 | 30,000 | 644,026 | 0.22 | 239,625 | 0.082 | 72.8 |
| CS-PK_30 kb-2 | 30,000 | 536,273 | 0.15 | 150,510 | 0.048 | 73.4 |
| CS-PK_40 kb-1 | 40,000 | 213,928 | 0.06 | 64,325 | 0.019 | 74.9 |
| CS-PK_40 kb-2 | 40,000 | 627,945 | 0.21 | 241,189 | 0.079 | 76.8 |
| CS-PK_40 kb-3 | 40,000 | 573,313 | 0.19 | 224,264 | 0.073 | 74.5 |
| RNA, Illumina 1 × 100 bp single-end reads | ||||||
| PK-Mid-flower | - | 37,835,287 | 3.8 | 25,687,331 | 2.3 | - |
| PK-Early-flower | - | 37,472,665 | 3.7 | 25,434,724 | 2.3 | - |
| PK-Pre-flower | - | 54,026,640 | 5.4 | 35,522,980 | 3.2 | - |
| PK-Shoot | - | 55,653,984 | 5.6 | 36,204,828 | 3.3 | - |
| PK-Stem | - | 60,353,149 | 6.0 | 39,274,463 | 3.5 | - |
| PK-Root | - | 37,374,640 | 3.7 | 24,904,927 | 2.2 | - |
| RNA, Illumina 2 × 100 bp paired-end reads | ||||||
| PK-subtracted1 | 180 | 110,483,894 | 22.1 | 64,525,082 | 11.6 | - |
| PK-subtracted2 | 180 | 82,190,044 | 16.4 | 46,291,148 | 8.3 | - |
| PK-subtracted3 | 180 | 105,737,119 | 21.1 | 61,974,962 | 11.2 | - |
| PK-subtracted4 | 180 | 48,599,953 | 9.7 | 26,505,457 | 4.8 | - |
