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Table 1 Purple Kush sequencing library statistics

From: The draft genome and transcriptome of Cannabis sativa

Library Insert size (bp) Raw No. Raw nt (Gb) Filtered No. Filtered nt (Gb) % in a) assembly
Genomic DNA, Illumina 2 × 100 bp paired-end reads
CS-PK_SIL-1a 181 143,951,601 28.0 124,499,863 23.8 82.2
CS-PK_SIL-1b 195 111,106,936 22.2 98,124,711 19.0 82.4
CS-PK_SIL-2a 313 93,774,355 18.8 81,421,333 15.3 84.7
CS-PK_SIL-3b 362 66,932,319 13.4 60,519,955 11.6 82.8
CS-PK_SIL-B 664 95,648,778 19.1 49,550,098 9.2 85.1
CS-PK_SIL-C 580 101,329,142 20.3 72,977,620 13.6 87.1
Genomic DNA, Illumina 2 × 44 bp mate pair reads
CS-PK_2 kb-1a 1,926 36,057,086 3.2 24,688,690 2.2 75.0
CS-PK_2 kb-1b 1,846 32,385,628 2.8 24,405,458 2.1 76.8
CS-PK_2 kb-2a 1,850 37,761,064 3.3 29,927,921 2.6 75.5
CS-PK_2 kb-2b 1,787 37,111,622 3.3 28,744,604 2.5 77.1
CS-PK_5 kb-1 4,721 36,182,230 3.2 27,377,398 2.4 77.5
CS-PK_5 kb-2 4,585 64,613,144 5.7 50,712,974 4.4 79.9
Genomic DNA, 454 mate pairs
CS-PK_8 kb-1 8,000 557,443 0.20 192,483 0.069 77.0
CS-PK_8 kb-2 8,000 484,033 0.17 176,405 0.063 74.5
CS-PK_8 kb-3 8,000 603,780 0.21 221,616 0.079 78.6
CS-PK_13 kb-1 13,000 430,642 0.11 96,503 0.030 75.1
CS-PK_20 kb-1 20,000 611,986 0.19 216,379 0.070 77.0
CS-PK_20 kb-2 20,000 575,618 0.21 228,811 0.081 77.0
CS-PK_30 kb-1 30,000 644,026 0.22 239,625 0.082 72.8
CS-PK_30 kb-2 30,000 536,273 0.15 150,510 0.048 73.4
CS-PK_40 kb-1 40,000 213,928 0.06 64,325 0.019 74.9
CS-PK_40 kb-2 40,000 627,945 0.21 241,189 0.079 76.8
CS-PK_40 kb-3 40,000 573,313 0.19 224,264 0.073 74.5
RNA, Illumina 1 × 100 bp single-end reads
PK-Mid-flower - 37,835,287 3.8 25,687,331 2.3 -
PK-Early-flower - 37,472,665 3.7 25,434,724 2.3 -
PK-Pre-flower - 54,026,640 5.4 35,522,980 3.2 -
PK-Shoot - 55,653,984 5.6 36,204,828 3.3 -
PK-Stem - 60,353,149 6.0 39,274,463 3.5 -
PK-Root - 37,374,640 3.7 24,904,927 2.2 -
RNA, Illumina 2 × 100 bp paired-end reads
PK-subtracted1 180 110,483,894 22.1 64,525,082 11.6 -
PK-subtracted2 180 82,190,044 16.4 46,291,148 8.3 -
PK-subtracted3 180 105,737,119 21.1 61,974,962 11.2 -
PK-subtracted4 180 48,599,953 9.7 26,505,457 4.8 -
  1. a) Percentage of genomic DNA reads that could be mapped back to the canSat3 genome assembly