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Table 5 Cross-platform correlation of relative abundances of E. coli lipidsa

From: A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language

  Statistical Orbitrap versus QSTARc Orbitrap versus MPIS QSTARd QSTAR versus MPIS QSTARe
Mode Estimatesb PE PG PE PG PE PG
MS Correlation coefficient 0.99 0.99 0.97 0.94 0.95 0.94
  Slope 0.95 1.14 1.0 0.85 1.03 0.89
MS/MS Correlation coefficient 0.99 0.99 0.97 0.94 0.95 0.94
  Slope 0.96 0.96 1.00 0.85 1.03 0.89
  1. aRelative abundances of PE and PG species are presented in Figure 10. bThe values of correlation coefficients R2 and slopes were calculated from corresponding scatter plots. cSpecies were quantified by LipidXplorer using MS and MS/MS spectra acquired independently on the Orbitrap and QSTAR, respectively. MS/MS experiments were performed in data-dependent acquisition mode. dSpecies were quantified by LipidXplorer using, respectively, MS and MS/MS spectra acquired at the Orbitrap machine. Relative abundances of individual species were correlated against corresponding relative abundances independently determined by MPIS on the QSTAR and LipidProfiler software. eSpecies were quantified by LipidXplorer using, respectively, MS and MS/MS spectra acquired on the QSTAR machine in data-dependent acquisition mode. Relative abundances of individual species were correlated with those determined by MPIS and LipidProfiler.