Secretome of A. benhamiae grown on keratin. (a) A. benhamiae grown on keratin particles. Cyan, fluorescence brightener 28-stained fungal hyphae; orange, keratin particle autofluorescence. Scale bar: 10 μm. (b) Two-dimensional gel of secreted A. benhamiae proteins obtained from culture supernatant after 48 h cultivation in a shaking flask with 0.9 g/l glucose and 10 g/l keratin. The apparent molecular mass of proteins and the pI range of the first dimension are indicated. Proteins were identified by mass spectrometry (matrix-assisted laser desorption/ionization-time of flight/time of flight (MALDI-TOF/TOF)). Identified proteins are given in Table S5 in Additional file 6. See also Additional file 1 for more details.