Transcriptome and proteome expression analysis of Gis2p targets. (a) Scatterplot depicting relative changes of protein levels (y-axis) compared to mRNA levels (x-axis). Gis2p targets are shown in red, non-targets in grey. (b) Boxplots depicting relative changes of mRNA levels in gis2Δ cells compared to wild-type cells (knockout (KO) RNA; colored in red), and mRNA (overexpressing (OE) RNA; grey) and protein (OE protein; blue) levels of cells overexpressing GIS2. Whiskers extend from the 10th to the 90th percentile. The distribution of all features is shown on top (all genes). Genes annotated to the GO terms indicated to the left were retrieved from the SGD, and the number of plotted Gis2p target mRNAs/proteins within the respective GO group is indicated in brackets. Asterisks refer to P-values determined in a Mann-Whitney test and Student's t-test with Welch's correction comparing the distribution of Gis2p target genes assigned to specified GO term with the distribution of all measured features: ***P < 0.001; **P < 0.01; *P < 0.05.